Phytohemagglutinin and concanavalin A activate hepatitis B virus in peripheral blood mononuclear cells of patients with chronic hepatitis B virus infection
Identifieur interne : 000382 ( France/Analysis ); précédent : 000381; suivant : 000383Phytohemagglutinin and concanavalin A activate hepatitis B virus in peripheral blood mononuclear cells of patients with chronic hepatitis B virus infection
Auteurs : Pascal Bouffard [France] ; Jean-Pierre Lamelin [France] ; Fabien Zoulim [France] ; Daniel Lepot [France] ; Christian Trepo [France]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 1992-08.
English descriptors
- Teeft :
- Assay, Blot assay, Bouffard, Cell proliferation, Chronic hepatitis, Cona, Hbsag, Hepatitis, Human leukocytes, Human pbmc, Hybridization, Korba, Level increase, Lymphocyte, Mitogen, Mitogen exposure, Nylon membrane, Pbmc, Pbmc culture, Pbmc proliferation, Peripheral blood, Replicative, Replicative forms, Sodium acetate, Southern blot analysis, Southern blot assay, Trepo, Unstimulated, Unstimulated cultures, Viral, Viral genome, Virology, Virus infection, Woodchuck.
Abstract
Peripheral blood mononuclear cells (PBMC) from 25 patients with chronic hepatitis B were tested for the presence of free monomeric hepatitis B virus (HBV) DNA migrating as a single 3.2 Kb band by Southern blot analysis. The PBMC were cultured for 7 days in the presence of phytohemagglutinin (PHA) or concanavalin A (ConA) both of which yielded a proliferative response. By contrast, both bacterial lipopolysaccharide (LPS) and interleukin 2 (IL2) failed to do so. Dot blot assays were used to monitor HBV DNA level increase within PBMC. Following mitogen exposure HBV DNA levels increased above pre‐stimulation levels in 19/25 PHA cultures, 6/15 ConA cultures, 1/15 LPS cultures, and 1/15 IL2 cultures. In 15 patients, Southern blot analysis was carried out before and after PHA exposure. In 13/15 cases, a single 3.2 Kb band was observed in un‐stimulated cultures as well as in PHA cultures even though PHA induced a HBV DNA increase. One case exhibited bands migrating faster than the 3.2 Kb signal, compatible with replicating intermediates and one case provided evidence of viral concatemers within PBMC after PHA stimulation. No HBV DNA was detected in the culture supernatants. The increase of HBV DNA level in PBMC induced by mitogen was strongly associated with an increase in HBV DNA expression (HBV RNA and HBs antigen). These studies indicate that HBV DNA present in human PBMC does represent a potential reservoir for infection with endogenous reactivation following PBMC activation. © 1992 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.1890370404
Affiliations:
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ISTEX:D35D1D0F4B79F61611224364E4B919A615C55CACLe document en format XML
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Lyon</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Zoulim, Fabien" sort="Zoulim, Fabien" uniqKey="Zoulim F" first="Fabien" last="Zoulim">Fabien Zoulim</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM U271, Lyon</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Lepot, Daniel" sort="Lepot, Daniel" uniqKey="Lepot D" first="Daniel" last="Lepot">Daniel Lepot</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM U271, Lyon</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old 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type="main">Journal of Medical Virology</title><title level="j" type="alt">JOURNAL OF MEDICAL VIROLOGY</title><idno type="ISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><imprint><biblScope unit="vol">37</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="255">255</biblScope><biblScope unit="page" to="262">262</biblScope><biblScope unit="page-count">8</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>New York</pubPlace><date type="published" when="1992-08">1992-08</date></imprint><idno type="ISSN">0146-6615</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Assay</term><term>Blot assay</term><term>Bouffard</term><term>Cell proliferation</term><term>Chronic hepatitis</term><term>Cona</term><term>Hbsag</term><term>Hepatitis</term><term>Human leukocytes</term><term>Human pbmc</term><term>Hybridization</term><term>Korba</term><term>Level increase</term><term>Lymphocyte</term><term>Mitogen</term><term>Mitogen exposure</term><term>Nylon membrane</term><term>Pbmc</term><term>Pbmc culture</term><term>Pbmc proliferation</term><term>Peripheral blood</term><term>Replicative</term><term>Replicative forms</term><term>Sodium acetate</term><term>Southern blot analysis</term><term>Southern blot assay</term><term>Trepo</term><term>Unstimulated</term><term>Unstimulated cultures</term><term>Viral</term><term>Viral genome</term><term>Virology</term><term>Virus infection</term><term>Woodchuck</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Peripheral blood mononuclear cells (PBMC) from 25 patients with chronic hepatitis B were tested for the presence of free monomeric hepatitis B virus (HBV) DNA migrating as a single 3.2 Kb band by Southern blot analysis. The PBMC were cultured for 7 days in the presence of phytohemagglutinin (PHA) or concanavalin A (ConA) both of which yielded a proliferative response. By contrast, both bacterial lipopolysaccharide (LPS) and interleukin 2 (IL2) failed to do so. Dot blot assays were used to monitor HBV DNA level increase within PBMC. Following mitogen exposure HBV DNA levels increased above pre‐stimulation levels in 19/25 PHA cultures, 6/15 ConA cultures, 1/15 LPS cultures, and 1/15 IL2 cultures. In 15 patients, Southern blot analysis was carried out before and after PHA exposure. In 13/15 cases, a single 3.2 Kb band was observed in un‐stimulated cultures as well as in PHA cultures even though PHA induced a HBV DNA increase. One case exhibited bands migrating faster than the 3.2 Kb signal, compatible with replicating intermediates and one case provided evidence of viral concatemers within PBMC after PHA stimulation. No HBV DNA was detected in the culture supernatants. The increase of HBV DNA level in PBMC induced by mitogen was strongly associated with an increase in HBV DNA expression (HBV RNA and HBs antigen). These studies indicate that HBV DNA present in human PBMC does represent a potential reservoir for infection with endogenous reactivation following PBMC activation. © 1992 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Rhône-Alpes</li></region><settlement><li>Lyon</li></settlement></list><tree><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Bouffard, Pascal" sort="Bouffard, Pascal" uniqKey="Bouffard P" first="Pascal" last="Bouffard">Pascal Bouffard</name></region><name sortKey="Lamelin, Jean Ierre" sort="Lamelin, Jean Ierre" uniqKey="Lamelin J" first="Jean-Pierre" last="Lamelin">Jean-Pierre Lamelin</name><name sortKey="Lepot, Daniel" sort="Lepot, Daniel" uniqKey="Lepot D" first="Daniel" last="Lepot">Daniel Lepot</name><name sortKey="Trepo, Christian" sort="Trepo, Christian" uniqKey="Trepo C" first="Christian" last="Trepo">Christian Trepo</name><name sortKey="Trepo, Christian" sort="Trepo, Christian" uniqKey="Trepo C" first="Christian" last="Trepo">Christian Trepo</name><name sortKey="Zoulim, Fabien" sort="Zoulim, Fabien" uniqKey="Zoulim F" first="Fabien" last="Zoulim">Fabien Zoulim</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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